Conference Week

 Intro

This week was entirely dedicated to poster making. Much of the time was spent organizing, reorganizing, writing, and formatting our poster. One of the things that was debated was whether or not sonorensis appears gram positive or gram negative on R2A.

Methods

Method for gram staining is exactly the same as always. Please refer to the procedure denoted in the first week of February's post.

Results & Discussion



 Shown above are gram stains of sonorensis, one sample grown in TGY and the other grown in R2A. Notably, the R2A sample was more spread out than that of TGY, showing the decreased plaque stability, though they do both appear gram positive. The debate of gram status may have come from using older samples for gram staining, or it could have simply been a contaminated sample of another cocci type bacteria which was mistaken for sonorensis in the sample.

Conclusion

With the ANAS conference in the rear-view, it is time to now look forward to possibly continuing this project, and to hopping back on the AMC project. It is highly likely that, again, a majority of time will be spent on RNA isolation and qPCR. Next steps will be discussing exactly what we will be moving on to, and the timeline.

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